Composite

Part:BBa_K5371101:Design

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-28)


ERG20-(GGGS)3-MRBBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1680
    Illegal SpeI site found at 2372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1680
    Illegal SpeI site found at 2372
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1680
    Illegal BglII site found at 2406
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1680
    Illegal SpeI site found at 2372
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1680
    Illegal SpeI site found at 2372
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For continuous and complete expression of ERG20-(GGGS)3-MRBBS, stop codon of ERG20 is deleted to prevent a premature stop in this case. Length of the linker is adjusted to avoid frameshift, which may cause faulty translation.


Source

The ERG20-(GGGS)3-MRBBS is composed of ERG20, (GGGS)3 linker, and MRBBS. The ERG20 comes from genome of Saccharomyces cerevisiae. The MRBBS comes from genome of (), which was synthesized in our project.

References